Pcrxl-topo
SpletpCR-XL-2-TOPO (linearized) Linearized vector with bound topoisomerase, for TOPO® cloning of long blunt-ended PCR products. Positive clones disrupt a lethal lacZα-ccdB … SpletpCRII-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer
Pcrxl-topo
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Splet26. avg. 2015 · The amplified PCR product was purified by agarose gel extraction using a QIAquick gel extraction kit (Qiagen) per the manufacturer's protocol. All RNA products were generated using T7 RNA polymerase and a T7 MEGAshortscript kit (Ambion) according to the manufacturer's instructions. Splet20. feb. 2024 · The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR …
Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 Splet01. okt. 2002 · The product of the inverse PCR was gel extracted, cloned with the TOPO TA Cloning kit with vector pCRXL-TOPO (Invitrogen), and fully sequenced. Primers NppkI-F and NppkI-R were subsequently designed to target the full-length Type I ppk (Table 2 ).
Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 SpletpCR-Blunt II-TOPO (linearized) Linearized vector for TOPO® cloning of blunt PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free …
SpletpCR-XL-TOPO Parental vector for TOPO® TA cloning of long PCR products. Vector Map (Click image to enlarge) Search our products with this vector backbone We offer a wide …
SpletpCR-XL-TOPO Parental vector for TOPO® TA cloning of long PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download … i am not my own getty lyricsSplet测序用 TOPO™ TA Cloning™ 试剂盒可提供高效、5 分钟的一步克隆策略（“TOPO™ 克隆”），用于将 Taq 聚合酶扩增 PCR 产物直接插入质粒载体中以进行测序。 每个试剂盒均使用含经过特殊设计的测序引物位点（可从各反应中获得更多的插入序列和更少的载体序列）的 pCR™4-TOPO™ TA 载体。 i am not my own for i have been made newSpletpCR2.1 TOPO Cloning PCR fragments, 3931 bp, f1 & pUC ori, lacZα, Amp r Kan r, screening by blue white colonies Invitrogen pCRXL TOPO Cloning large PCR fragments (3–10 kb), 3519 bp, pUC ori, lacZα, Zeocin r Kan r, cedB lethal gene, screening by blue white colonies Invitrogen pKHKS403 mom harriet dad m15 e9 and siblings fan clubSpletThe TOPO XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and … i am not my hair bookSpletIntroduction The pCR®T7 TOPO® TA Expression Kits provide a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase … i am not my own bible verseSpletpCR4-TOPO. Parental sequencing vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. Download Plasmid. Download SnapGene Viewer. Explore Over 2.7k Plasmids: I.M.A.G.E. Consortium Plasmids More Plasmid Sets. mom handwriting tattooSpletThe TOPO™ XL-2 Complete PCR Cloning Kit provides a highly efficient one-step method ("TOPO™ Cloning") for the cloning of extra long PCR products generated by the … mom harriet dad m154 and siblings club