Immortalized human corneal epithelial cells
WitrynaHuman corneal epithelial cells (HCEpC) have been used as models for studying corneal damage and reconstruction, re-epithelialization of the eye following surgery, … WitrynaCorneal epithelial cells also express a high level of aldehyde dehydrogenase to protect them against UV- and 4-hydroxynonenal-induced cellular damage. The renewal of …
Immortalized human corneal epithelial cells
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Witryna15 paź 2010 · Purpose: To compare the global gene expression profile of stratified epithelia generated in vitro using simian virus 40 (SV40) immortalized human corneal epithelial cells with the previously reported gene expression of normal human corneal epithelia. Methods: Immortalized cells expanded in submerged culture were grown … Witryna11 kwi 2024 · 3.1.Corneal epithelial barrier models. As the primary physical barrier, the corneal epithelium constantly maintains both the metabolism and homeostasis of the human ocular surface through transporting nutrients and metabolites between tear film and corneal stroma [65, 66].This barrier also blocks harmful organisms and …
WitrynaNormal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. ... in which an HCE three … Witryna1 kwi 2008 · Methods Immortalized human corneal epithelial cells were grown to confluency on curved cellulose filters for seven days, and were then differentiated and stratified using an air-liquid interface ...
WitrynaThis result in a small cell yield for experiments such as the eye irritancy test represents a serious problem for human and animal corneal epithelial research. In the present … WitrynaPurpose: To develop an in vitro model of human corneal epithelium that can be propagated in serum-free medium that is tissue specific, species specific, and continuously available. Methods: Primary explant cultures from human cadaver donor corneas were generated and subsequently infected with Adeno 12-SV40 (Ad12 …
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Witryna3 kwi 2024 · The cytotoxicity of benzalkonium chloride (BAC) and disodium edetate (EDTA) was evaluated in vitro in rabbit corneal epithelial primary cells and in the … cb鳥栖フェリシアWitrynaDescription. Human corneal epithelial cells are the main components of the human cornea. The cornea is a unique transparent tissue, without blood vessels, and with … cc002 ミネベアWitryna7 maj 2024 · The immortalized human-derived corneal epithelial cells expressing SV40 were maintained in a cell culture medium supplemented with serum (Fig. 5b, … cc01bl2 オリエンタルWitrynaImmortalized Human Corneal Epithelial Cell Line (IM-HCEpiC) has been developed by immortalizing primary human corneal epithelial cells with SV40 Large T antigen. … cc-01 リダクションギア 取り付けWitrynaIn this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that … cc-01 ホイールベース 延長Witryna23 maj 2024 · Purpose Ocular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily applied. Histatin-1 … cc001-rs4 オリエンタルWitrynaThe Human Corneal Epithelial Cells are cryopreserved as secondary cells. The cells have been isolated from human corneal tissue and expanded twice in culture vessels … cc-01シャーシ