Web(C) Ubiquitin IP with the FK2 antibody from the cells expressing the indicated proteins, followed by IB with FK2 and the pan-ubiquitin antibody #3933. (D) IP using the HA antibody from the cells co-expressing HA-ubiquitin with the indicated proteins, followed by IB with the HA and pan-ubiquitin antibodies. WebMay 15, 2005 · Separation of ubiquitinated Syk using an antiubiquitin antibody. Washed platelets were activated with convulxin (100 ng/mL) for 15 seconds. Cells were lysed with RIPA buffer and treated with FK2 antibody for 1 hour at 4°C, followed by Protein A/G PLUS-agarose for an additional hour. Supernatants were treated with anti-Syk using the …
Cyclin-Dependent Kinase 5 Regulates PSD-95 Ubiquitination in …
WebGeneral description. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated proteins. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated ... WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin … can 18 bar fit a husky 435 chainsaw
Multiubiquitin Chain Monoclonal Antibody (Clone FK2)
WebImmunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ … WebDownload Zoom This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References Data Sheet … WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be … can 18x 87 be factored