Cyto-id staining

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August undefined, 2024

WebWe first measured autophagy in splenic B cell subsets using immunofluorescence Cyto-ID to detect autophagosomes by flow cytometry. We found autophagy to be higher in T2 MZP, MZB, PC, and IgM + PC cells, compared to FOB which also correlates with their relative CD36 expression ( Figure 3A ). WebJan 3, 2024 · Cyto-ID staining flow cytometry was used to detect autophagy compartments induced by autophagy activators with or without CQ (60 nM) pretreatment ( P < 0.05 by t test). e Cyto-ID fluorescence intensities of DMSO, EBSS, RAPA, and GDC-0152 Full size image Autophagy promotes GDC-0152-induced apoptosis in HL-60 cells

An antibody for analysis of autophagy induction Nature …

WebCyto-ID® Autophagy Detection Kit can measure autophagic vacuoles and monitors autophagic flux in live cells using a "novel dye" that selectively labels autophagic vacuoles. WebFeb 7, 2024 · (E) Cyto-ID staining of OV202 NTC and Sh1 cells in control, EBSS and EBSS + Baf treated cells. Cells were grown in either regular media or EBSS to induce autophagy. Cells were grown in either ... great wall essay

Cell damage evaluation by intelligent imaging flow cytometry

WebCyto-ID Green Dye Staining Protocol. Cyto-ID Green dye (8 µl) is mixed with 4 ml 1X buffer. Cell sample is centrifuged and resuspended into 200 µl of prepared Cyto-ID Green dye. Cell sample is incubated for 30 min at … WebJan 1, 2014 · The intensity of Cyto-ID fluorescence in RTCs did not increase after incubation with rapamycin for 3 h compared to the vehicle control or even exhibited a … WebCYTO-ID ® Autophagy detection kit 2.0 Brighter, more photostable dye specifically stains autophagic vesicles Rapid, no transfection required … great wall enfield ct menu

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CYTO-ID ® Autophagy detection kit - Enzo Life Sciences, …

WebDec 15, 2015 · Phosphatidyl serine exposure was detected by Annexin V staining, cell viability by propidium iodide (PI) and SYTO 16 staining, and CYTO-ID Green was used … WebCyto-ID assay is based on the usage of a specific dye that selectively stains autophagic compartments and therefore allows determination of autophagic flux as accumulation of stained compartments in basic or activated conditions [rapamycin (15 µmol/L-), PP242 (1-5 µmol/L) or Hanks’ Balanced Salt Solution containing 6 mmol/L glucose (starvation … florida gator rainbow sandalsWebMar 26, 2024 · Removing excessive platelets using acoustofluidic methods receives extensive attention due to their high efficiency and high yield. While the damage to the remaining cells, such as erythrocytes and leukocytes is yet evaluated. Existing cell damage evaluation methods usually require cell staining, which are time-consuming and labor … greatwall enterprises limited

"WebWe first verified that the Cyto-ID dye specifically labels autophagic compartments with minimal staining of lysosomes and endosomes. We then developed a new Cyto-ID … " - Cyto-id staining

Cyto-id staining

SYTO™ 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in …

Webtook advantage of Cyto-ID, a uorescent dye for selective staining of autophagosomes [45–47], in two ways: First, we conducted double-labeling ow cytometry with Cyto-ID and the live-cell-permeant DNA marker DRAQ5, allowing for simultaneous monitoring of autophagy and cell cycle phases. Second, we developed a Cyto-ID-based ow-cyto- WebJul 21, 2024 · Autophagosome staining was then performed using the Cyto-ID reagent, and Hoechst 33342 as a counterstain. 2 × 2 composite images were taken on a Zeiss AxioObserver motorized microscope at a ×10 ...

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http://biopioneer.com.tw/?p=41115 WebJan 30, 2013 · Determination of autophagy (Cyto-ID staining) Flow cytometric analysis of Cyto-ID Green Detection Reagent stained cells was performed according to manufacturer's protocol (Cyto-ID Autophagy Detection Kit, ENZ-51031-K200, Enzo Life Sciences). In brief, harvested cells were washed with PBS, stained with Cyto-ID in indicator-free medium …

WebJan 1, 2014 · The intensity of Cyto-ID fluorescence in RTCs did not increase after incubation with rapamycin for 3 h compared to the vehicle control or even exhibited a tendency to decrease (Fig. 5, a and e). ... WebApr 14, 2024 · For CYTO-ID staining, cells were incubated for 30 min in N2B27 containing 2 μl/ml of CYTO-ID Green. Cells were then washed and fixed with 4% paraformaldehyde and processed for immunofluorescence imaging as above. Wide-field microscopes used: Leica DMRD upright fluorescence microscope fitted with a Leica DFC450 C camera 5 …

WebNov 5, 2024 · After staining with Cyto-ID, autophagosomes were quantified measuring an increase in green fluorescence (FL1) using flow cytometry (** p < 0.01, ***p < 0.001 compared to the serum only group). WebMar 3, 2024 · The CYTO-ID dye is known to stain autophagic vesicles ( 24 ), and we have previously demonstrated similar results obtained by analyses of CYTO-ID staining and by detection of LC3-II ( 8 ). Forskolin enhanced the IR-induced autophagy, as revealed both by CYTO-ID staining and LC3-II detection ( Fig. 1A – D ).

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WebWe first verified that the Cyto-ID dye specifically labels autophagic compartments with minimal staining of lysosomes and endosomes. We then developed a new Cyto-ID fluorescence spectrophotometric assay that makes it possible to estimate autophagy flux based on measurements of the Cyto-ID-stained autophagic compartments. florida gator motorcycleWebJan 16, 2016 · Cyto-ID Green concentration contains 1 μl of Cyto-ID Green Detection Reagent in 1 ml cell culture medium. It was then mixed well and incubated for 30 min under standard tissue culture conditions at 37 °C, 5 % CO 2 in the dark. At the end of staining procedure, the Cyto-ID containing medium was washed with PBS. florida gator predictions 2023WebJun 26, 2024 · LysoTracker + (top) and CYTO-ID + (bottom) vesicles are shown in the background of MAP2 neuronal staining (n = 3 biological replicates). Scale bars are 25 μm. Scale bars are 25 μm. Source data ... great wall epsomWebCyto-Last™ Buffer is specially formulated for the storage of cytokine producing cells. When used, staining of intracellular and/or extracellular targets for flow cytometric analysis can be delayed for up to two weeks. Cells should be stored at 4°C during this time. This unique buffer ensures cells maintain a background staining signal equal ... greatwall engine assy factoryWebIn this work, the Cyto-ID dye is shown to colocalize with RFP-LC3 in starved HeLa cells, which further validate the specificity of the dye. The developed image-based autophagy … florida gators 2023 baseball scheduleWebNov 25, 2024 · Co-staining of endogenous pATG16L1 s278 and LC3B revealed a partial colocalization and upregulation of puncta with both … florida gators away jerseysWebUsing Cyto-Tracers, cellular structures can be visualized in real-time by fluorescence microscopy without the use of chemical staining. You can also monitor the dynamic movement of a protein-of-interest in relationship to a given subcellular compartment or structure by labeling the protein-of-interest with separate fluorescent proteins. great wall erie