Cisplatin ic50 a549

WebMar 22, 2024 · The IC50 (drug concentration producing 50% growth inhibition) was calculated with GraphPad Prism Version 5.0 Software. Cell proliferation assay Cell … WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells …

An IC50 curve for MCF7 cells treated with cisplatin. Percentage …

WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were performed to reveal the relative mobility. The line graphs show the mean ± SD of three scratches from one representative experiment. WebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 ... USA; CRM‐CCL‐185), A549 cisplatin‐resistant (A549/DDP; National Infrastructure of Cell Line Resource, Beijing, China; 1101HUM‐PUMC000519), and human lung bronchial epithelial 16HBE cells (Shanghai Fuheng Biotechnology Co., Ltd. China; … diamond painting roller tool https://mubsn.com

TAZ inhibition restores sensitivity of cisplatin via AKT/mTOR

WebImmunofluorescence results elucidated that cellular ROS level in Cisplatin-induced A549 cells increases in a dose-dependent manner. Both immunofluorescence and flow cytometry results revealed that the apoptotic rate of A549 cells increases with the elevation of Cisplatin dose. WebJan 3, 2003 · The concentration of Gleevec to inhibit 50% cell growth (IC50) was estimated to be around 2–3 μM (Figure 1), whereas the IC50 of cisplatin on A549 cells was … WebMar 17, 2024 · To establish the cisplatin-resistant cell line, A549-R, A549 cells were treated with a stepwise increasing concentration of cisplatin (Sigma-Aldrich, USA) as previously described. 29, 30 The CAFs and normal fibroblasts (NFs) were isolated from NSCLC tissues and adjacent normal tissues by primary culture, as previously described, respectively. … cirs regulatory

Metformin enhances the radiosensitizing effect of cisplatin in …

Category:Cisplatin Induces a Mitochondrial-ROS Response That Contributes …

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Cisplatin ic50 a549

Metformin impairs cisplatin resistance effects in A549 lung cancer ...

WebDec 1, 2024 · microRNA IC50 inhibit concentration by 50% Keywords Colorectal neoplasiaa Drug resistance Oxaliplatin ABCB1 GSTP1 miRNA 1. Introduction Colorectal cancer is one of the major fatal malignancies, which is the third and second most prevalent cancer in males and females respectively. WebWith the treatment prolongation of 4 μmol/L Cisplatin in A549 cells, Bcl-2 and caspase-3 were downregulated, while BAD upregulated. Conclusions: Cisplatin treatment induces …

Cisplatin ic50 a549

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WebJul 17, 2024 · Importantly, we show that treatment of cancer cells with concentrations of cisplatin that result in 50% effect (i.e., IC50) in multiwell plate assays trigger the emergence of growth arrested cells that exhibit highly enlarged morphology, remain viable and adherent to the culture dish, and metabolize the tetrazolium salt 3-(4,5-dimethylthiazol-2 ... WebIC 50 values for cisplatin, curcuminoid, and combination treatments in A549 cell culture. Standard deviation values are provided after the ± symbol for each inhibitory concentration value. Cell...

WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug …

WebJan 3, 2003 · We have also shown that A549 cells are resistant to cisplatin treatment (IC50 64 microM). Addition of Gleevec to the A549 cells treated with cisplatin resulted in a synergistic cell killing effect, suggesting that Gleevec can potentiate the … WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …

WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated …

WebMar 22, 2024 · The drug’s IC 50 values indicated that the cells had acquired a stable CDDP-resistance phenotype, with the IC 50 values of the A549/DDP cells remaining … diamond painting round vs squareWebJul 23, 2015 · The IC50 of cisplatin for A549 and SPC-A1 cells under hypoxia was 3.38-fold and 1.57-fold higher as compared to that under normoxia respectively as showing by the … cirstea ageWebFeb 28, 2024 · Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL-7702) cells. ... (IC50 = 3.87 μM), complex 40 (IC 50 = 0.91 μM), and cisplatin (IC 50 = 50.12 μM), all of which showed lower cytotoxic effects against HL-7702 compared with the standard approved regimen … cirs syndrome treatmentWebIC 50 s of oxaliplatin in HCT116 and A549 cancer cells in the absence and presence of WLR treatment Source publication +2 Aqueous extract of Lithospermi radix attenuates oxaliplatin-induced... cirsscheduling hasa.orgWebNational Center for Biotechnology Information cirstea andreeaWebNov 19, 2013 · A549 cells were exposed to cisplatin (CDDP) at an IC50 dose (12 µM) or a range of carboplatin (CBCDA) doses and total intracellular ROS levels were measured … cirstea boyfriendWebLung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated … cir state of alaska